Biotage® Isolera LS Systems

Isolera™ Spektra LS flash systems are automated purification systems designed upto 150g of sample per run, whether natural product extracts or other organic compound mixtures from chemistry.

Biotage® Isolera LS is the definitive flash chromatography system for reliable scaling up from grams to multi, even hundred gram scale purification, using the industry standard original Isolera interface. Enjoy all of the sophisticated software features of laboratory scale systems in a robust instrument built for scale-up labs. Isolera Spektra LS adds an integrated pump assisted loading mechanism for larger samples, and a funnel rack collection option which allows samples to be collected in larger fraction collection vessels.


Benefits

Biotage® Isolera LS is the definitive flash chromatography system for reliable scaling up from grams to multi, even hundred gram scale purification, using the industry standard original Isolera interface. Enjoy all of the sophisticated software features of laboratory scale systems in a robust instrument built for scale-up labs. Isolera Spektra LS adds an integrated pump assisted loading mechanism for larger samples, and a funnel rack collection option which allows samples to be collected in larger fraction collection vessels.

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Up to 30% solvent savings with Gradient Optimization “GO”

Step-gradients can be short and powerful providing separations for one or more compounds but can be challenging to develop and optimize. Isolera Spektra provides gradient optimization through new, patent-pending TLC-to-Step Gradient technology. Using solvent and TLC Rf values, Isolera Spektra builds a gradient to separate all the compounds (up to 6) in the sample. This new technology will also provide cartridge selection guidance based on the cartridge loading capacity and purification speed. The step gradient can also be used to isolate a targeted compound reducing run time and solvent use further.

Improve fraction and compound purity with λ-All detection and PDA spectral analysis

PDA scanning and λ-All technology detects any UV absorbing compound eluting from a flash cartridge while measuring and displaying each eluting compound’s individual UV spectrum. Combined with novel baseline rise correction, yield losses to improper wavelength selection and large fraction volumes are no longer a concern. The PDA spectra can be reviewed and used to determine fraction purity eliminating the need for post-flash purity analysis.

Elute complex samples with the Quatro-binary gradient

Use up to four solvents in a single gradient to easily purify samples with diverse polarity. With Quatro-binary gradient capability traditional binary gradients with a limited polarity range can be adjusted to elute very lipophilic and highly polar compounds within a single purification or compound solubility during the separation by adding a constant amount of a co-solvent, acid, or base with the Isolera advanced pump.

Enhance productivity with on-the-fly editing

Isolera™ methods can easily be edited either in front of the Isolera or from the comfort of your office. Edit the gradient (click & drag points AND segments), flow rate, collection volume, fraction wavelengths and modes, and add more collection racks if you need to – all while the run is in progress. Gradient changes can now be made with a simplified graphical interface or through a table layout.




Isolera LS System Part numbers

Part No. Description Pack Size
ISO-1LSV Isolera™ LS, One Channel, Extended Collection Bed, 200-400 nm Detector 1
ISO-1LSW Isolera™ LS, One Channel, Extended Collection Bed, 200-800 nm UV-VIS Detector 1



Development and SNAP XL Cartridges Selector

Part No. Description Pack Size
FSN0-0454-1800 Biotage® SNAP Isolute NH2, 1800g 1/cs 1
FSN0-0454-0900 Biotage® SNAP Isolute NH2, 900g 1/cs 1
FSL0-1118-0120 Biotage® SNAP KP-C18-HS 120 g, 2/cs 2
FSL0-1118-1850 Biotage® SNAP KP-C18-HS 1850 g, 1/cs 1
FSL0-1118-0400 Biotage® SNAP KP-C18-HS 400 g, 1/cs 1
FSL0-1118-0060 Biotage® SNAP KP-C18-HS 60 g, 2/cs 2
FSL0-1118-0950 Biotage® SNAP KP-C18-HS 950 g, 1/cs 1
FSN0-0909-0110 Biotage® SNAP KP-NH 110 g, 10/cs 10
FSN0-0909-1800 Biotage® SNAP KP-NH 1800 g, 1/cs 1
FSN0-0909-0375 Biotage® SNAP KP-NH 375 g, 1/cs 1
FSN0-0909-0055 Biotage® SNAP KP-NH 55 g, 10/cs 10
FSN0-0909-0900 Biotage® SNAP KP-NH 900 g, 1/cs 1
FSK0-1107-0100 Biotage® SNAP KP-SIL 100 g, 20/cs 20
FSK0-1107-1500 Biotage® SNAP KP-SIL 1500 g, 2/cs 2
FSK0-1107-0340 Biotage® SNAP KP-SIL 340 g, 6/cs 6
FSK0-1107-0050 Biotage® SNAP KP-SIL 50 g, 20/cs 20
FSK0-1107-0750 Biotage® SNAP KP-SIL 750 g, 2/cs 2
FSUL-0442-0100 Biotage® SNAP Ultra 100 g, 20/cs 20
FSUL-0442-0340 Biotage® SNAP Ultra 340 g, 6/cs 6
FSUL-0442-0050 Biotage® SNAP Ultra 50 g, 20/cs 20
FSUL-0401-0120 Biotage® SNAP Ultra C18 120 g, 2/cs 2
FSUL-0401-1850 Biotage® SNAP Ultra C18 1850 g, 1/cs 1
FSUL-0401-0400 Biotage® SNAP Ultra C18 400 g, 1/cs 1
FSUL-0401-0060 Biotage® SNAP Ultra C18 60 g, 2/cs 2
FSUL-0401-0950 Biotage® SNAP Ultra C18 950 g, 1/cs 1
FSN0-0454-0375 SNAP Cartridge, Isolute Flash-NH2, 375g 1
YT6-2030-0050 SNAP Cartridges, (20) Packed with HP-20 50g 20
YT6-2030-0100 SNAP Cartridges, (20) Packed with HP-20, 100g 20
YT6-2530-0100 SNAP Cartridges, (20) Packed with HP-20SS, 100g 20
YT6-2530-0050 SNAP Cartridges, (20) Packed with HP-20SS, 50g 20
YT6-2030-0340 SNAP Cartridges, (6) Packed with HP-20, 340g 6
YT6-2530-0340 SNAP Cartridges, (6) Packed with HP-20SS, 340g 6
FSN0-0454-0110 SNAP Cartridges, Isolute Flash-NH2 110g, 10/pk 10
FSN0-0454-0055 SNAP Cartridges, Isolute Flash-NH2, 55g, 10/pk 10
FSK0-0445-0340 SNAP Cartridges, KP-Sphere, 340g, 6/cs 6
FSAC-4021-0100 SNAP Flash-AC, 100g cartridge 20pk 20
FSAC-4021-0340 SNAP Flash-AC, 340g cartridge 6pk 6
FSUL-0442-1500 Snap Ultra 1500g, Pack of 2 2
FSUL-0442-0750 Snap Ultra 750g, Pack of 2 2
FSUL-0443-1500 SNAP XL 1500g with Biotage Sfär HC silica 2
FSUL-0443-0750 SNAP XL 750g with Biotage Sfär HC silica 2



Specifications

Pipette Calibration Notifications  WEIGHT     40 kg (88 lbs) typical, depending on configuration

Dimensions 3D  DIMENSIONS     565 mm (22”) x 596 mm (23.5”) x 497 mm (19.6”) (expanded bed)

Flow Rate  FLOW RATE RANGE     50 – 500 mL/min

search  FRACTION COLLECTION MODES     Volume, threshold, threshold with volume, low slope, medium slope

ic remove red eye 24px@4x  FLOW CELL PATH LENGTH     0.3 mm

Flow  SOLVENT DELIVERY     Two constant volume (11-mL) electric HPFC pumps

Electrical Supply  POWER REQUIREMENTS     100 – 240 VAC, 50/60 Hz, 4.0 A

Fraction Volume  COLLECTION VESSELS     Test tubes (13, 16, 18, and 25 mm) and bottles (120 mL, 240 mL)

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UV DETECTION     Choice of variable wavelength (200–400 nm) or UV-VIS (200–800 nm

Sunglasses UV  UV COLLECTION MODES     Single/dual/λ-All wavelengths (variable UV and UV-VIS)