Biotage® Sfär

Sfär for Spherical, Biotage for Quality

Sfär is the Swedish word for ”sphere”, and the name of our columns reflects the fact that we have made spherical silica a standard due to its higher surface area and higher loading capacity. Reliable and flexible, Biotage® Sfär columns deliver larger loading capacities, tighter elution bands and purer fractions than traditional flash columns.

Sfär columns are available in different sizes and media and with specific characteristics.

The HC in the name stands forHigh Capacity and it refers both to loading capacity and performance – this derives not only from spherical silica, but also from smaller particles size (20 µ). Combined, this high surface, small particle silica improves purification performance while reducing the amount of silica and solvent required for purification. This means you can choose between running your separation with a smaller column, using less silica and less solvent, or you can increase your loading capacity and obtain a higher amount of your compound at the end.

The D stands for Duo (i.e. Two), as Biotage Sfär gives you the possibility of external and internal liquid and dry loading, you can choose among six different ways to load your sample on the columns based on the sample characteristics.

Our Columns

The selection of a column can be based on the type of flash chromatography (normal phase, reversed phase, specialty phase) and the size of the molecules. First of all, though, you should decide whether you want to follow a traditional approach or trust the Biotage way to make your chemistry simpler, faster, greener. In one word, better.

Whether you plan to use liquid loading only on a closed column, or liquid and dry internal loading on a open column, use the filters below to find the most suitable columns for your need.


Biotage developed and introduced pre-packed columns for flash purification in 1994. Today our broad selection of columns enables professionals to choose the solution which best suits their purification needs. Biotage® Sfär is a complete portfolio of flash columns, available in a large variety of sizes from 5 g to 350 g and in a range of media types, so you can purify milligrams to multi-grams of your valuable compounds quickly and easily.


Quality First

The process of packing our columns has make huge steps since the first pre-packed column more than 20 years ago, reaching very high quality standards that make your projects move faster because the separation runs the first time. All Sfär columns come with a printed, unique identifier to enable recognition and categorization based on the application. Thanks to the unique QR code on every column, it is extremely quick and easy to load the column data into the instrument when used with Biotage® Selekt. This will immediately set the ideal conditions in terms of flow and pressure to run the column. Sfär columns can be run up to a pressure from 12 bar (Duo) to 20 bar (Closed) depending on the size, higher that many other commercially available columns.

The Greenest Columns

Chemistry, by its very nature, involves the use of chemicals that can be harmful, toxic and potentially damaging to the environment. Drug discovery currently has a large and expensive environmental footprint so finding a solution is good for both the chemist and the environment. A first step to a greener purification is using a column with a higher loading capacity, so you can purify the same amount of sample on a smaller column and hence use lower flow rates and less solvent; and ultimately finish the job quicker. This will make your whole process greener, less expensive and also more efficient. The best way to experience that is with the Biotage® Sfär HC (High Capacity).

The Most Flexible Columns

The unique design of Biotage® Sfär makes it possible to load your columns in six different ways, including three different dry loading options thanks to the patented Biotage Samplet® and DLV. For your difficult separations, when you want to utilize dry loading in the most convenient way, take advantage of the orange flower shaped cap of Biotage® Sfär D (Duo). When used with Biotage® Selekt, flash purification system, the instrument will suggest the optimal cartridge for your conditions and will help you in optimizing your separation even further.